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This particular Assignment references the syllabus chosen for the subject of Biochemistry, for the January 2024 - December 2024 session. The code for the assignment is BBCS-183 and it is often used by students who are enrolled in the BSC (Honours) Degree.
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1. Define the following terms:
i) Mole
ii) Normality
iii) Zwiterions
iv) Stock shift
v) Reverse osmosis
2. Calculate how much quantity of substance would be needed to make following solutions:
(i) 10mL of 0.05M NaOH (ii) 10mL of 0.5N KOH
(iii) 0.9% NaCl (iv) 5mL of 0.5% HCL
3. (a) Explain how does distillation method purify impurities from water.
(b) Write the Laboratory safety rules.
4. (a) Describe the instrumentation of spectrophotometry.
(b) What is Beer-Lambert Law? Explain
5. Write short notes on the following:
(a) Role of virtual lab in Biochemistry
(b) Buffer
1. Define the following terms:
i) Molarity
ii) pH
iii) Distilled water
iv) Molar extinction coefficient
v) Filtration
2. Calculate how much quantity of substance would be needed to make following solutions:
(i) 50mL of 0.05M NaCl (ii) 10mL of 0.5N HCl
(iii) 1% H2SO4 (iv) 20mL of 0.5% NaOH
3. (a) What is ion exchange method? Explain how it purify impurities from water.
(b) Write the principle and procedure of fluorescence spectroscopy.
4. (a) State the principle of spectrophotometry:
(b) Discuss the laboratory safety rules and safety symbols?
5. Write short notes on the following:
(a) Virtual lab in Biochemistry
(b) pH meter and its calibration
1. निम्नलिखित को परिभाषित कीजिये ।
(i) मोल
(ii) नॉर्मलता
(iii) ज्वटेरियन्स
(iv) स्टॉक शिफ्ट
(v) रिवर्स ऑस्मोसिस
2. गणना कीजिए कि निम्नलिखित विलयनों को बनाने के लिए कितनी मात्रा में पदार्थ की आवश्यकता होगी:
(a) 10ml of 0.05M सोडियम हाइड्रॉक्साइड
(b) 10ml of 0.5N सोडियम हाइड्रॉक्साइड
(c) 0.9% क्लोराइड
(d) 5ml of 0.5% हाइड्रोक्लोरिक एसिड
3. (क) बताएं कि आसवन विधि पानी से अशुद्धियों को कैसे शुद्ध करता है।
(ख) प्रयोगशाला सुरक्षा नियमों को लिखिए।
4. (क) स्पेक्ट्रोफोटोमेट्री के उपकरणों का वर्णन कीजिये :
(ख) बीयर लॅबर्ट नियम क्या है? समझाइए ।
5. निम्नलिखित पर संक्षिप्त टिप्पणी कीजिये :
(क) जैव रसायन में आभासी प्रयोगशाला की भूमिका
(ख) बफर
1. निम्नलिखित को परिभाषित कीजिये ।
(i) मोलरता
(ii) पीएच
(iii) आसुत जल
(iv) ग्रामअणुक विलोप गुणांक
(v) निस्पंदन
2. गणना कीजिए कि निम्नलिखित विलयनों को बनाने के लिए कितनी मात्रा में पदार्थ की आवश्यकता होगी:
(a) 50ml of 0.05M सोडियम क्लोराइड
(b) 10ml of 0.5N हाइड्रोक्लोरिक एसिड
(c) 1% सल्फ्युरिक एसिड
(d) 20mL of 0.5% सोडियम हाइड्रॉक्साइड
3. (क) आयन विनिमय विधि क्या है? बताएं कि यह पानी से अशुद्धियों को कैसे शुद्ध करता है।
(ख) प्रतिदीप्ति स्पेक्ट्रोस्कोपी के सिद्धांत और प्रक्रिया को लिखिए ।
4. (क) स्पेक्ट्रोफोटोमेट्री के सिद्धांत को बताइए
(ख) प्रयोगशाला सुरक्षा नियमों और सुरक्षा प्रतीकों की व्याख्या करें ।
5. निम्नलिखित पर संक्षिप्त टिप्पणी कीजिये :
(क) जैव रसायन में आभासी प्रयोगशाला
(ख) पीएच मीटर और इसका अंशांकन
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