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This particular Assignment references the syllabus chosen for the subject of Biochemistry, for the January 2024 - December 2024 session. The code for the assignment is BBCCT-123 and it is often used by students who are enrolled in the BSC (Honours) Degree.
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1. A) What are promoter sequences. Explain their significance in prokaryotic transcription.
B) Draw and explain the structural organization of a gene.
2. A) Describe transcription bubble formation in prokaryotes.
B) What are inhibitors? List prokaryotic transcription inhibitors and describe any two of them.
3. Write a note on RNA polymerase I mediated transcription in eukaryotes.
4. A) Give a comparative account on eukaryotic and prokaryotic transcription.
B) Explain the mechanism of mRNA capping and its biological significance.
5. A) Define mRNA splicing. Describe spliceosome mediated splicing.
B) Differentiate between alternative and trans splicing.
6. A) Elaborate homing and retrohoming mechanisms.
B) Illustrate the structure of transfer RNA and its functions.
7. What is genetic code? Explain Khorana experimental contributions in understanding genetic code. Give the salient features of genetic code.
8. Write a detailed note on eukaryotic translation.
9. A) What is antibiotic? Give the details of antibiotics used in medicine.
B) Differentiate between negative and positive gene regulation.
10. A) Explain how SOS response help DNA in repairing the damage caused by external agents.
B) Describe the synthesis and function of miRNA
1. A) Write a brief note on prokaryotic RNA Polymerases.
B) Explain the DNA foot printing technique.
2. A) Describe Rho-dependent and Rho-independent termination of transcription.
3. A) Give a detailed account of prokaryotic transcription inhibitors.
B) With the help of neatly labelled diagrams explain the functions of ‘cap’ and “Poly-A” tail of eukaryotic m-RNA.
4. A) What is RNA splicing? Explain the steps involved spilceosome machinery with a neat diagram?
B) Explain riboswitches with the help of their structures.
5. Write a detailed note on salient features of genetic code with suitable diagrams.
6. A) Explain the structure and functions of tRNA using a neatly labelled diagram.
B) Enlist protein translation factors.
7. Describe the protein translation in prokaryotes with the help of a labelled diagram.
8. Write a detailed note on role of activators and repressors in gene expression and regulation with relevant examples.
9. A) Describe the concept of ‘operons’ in gene expression.
B) With the help of a neatly labelled diagram explaining regulation of “lac operon”.
10. A) Explain the physiology and consequences of SOS responses in prokaryotes.
B) Write a short note on hetero chromatin and euchromatin.
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