PART-(A)
1. Write in brief on the following:
a) Prosthetic Group
b) Bisubstrate Reactions
c) Enzyme Specificity
d) Mixed inhibition
2. a) Explain Transition state theory of enzyme catalyzed reactions.
b) What is the effect of metal ions on enzyme activity?
3. Give an overview of IUBMB system of enzyme classification.
4. Differentiate between:
a) Ping Pong Bi-Bi and Random Sequential Mechanism
b) Kcat and Turnover Number
5. Derive Michaelis-Menten equation for a non-competitive inhibitor reactions.
PART-(B)
6. How compartmentation helps to control metabolic pathways?
7. Write note on any two of the following:
a) Flavin nucleotides
b) Diagnostic Enzymes
c) Effect of pH on immobilized enzyme
8. Discuss the Fatty Acyl Synthase complex.
9. Differentiate between the following:
i) Metal Activated enzymes and Metalloenzymes
ii) Gel Entrapment and Fibre entrapement method of enzyme immobolization
10. Discuss application of enzymes in the following:
i) Food industry
ii) Fruit Juice and Wine industry
PART-(A)
1. Write short note on the following:
a) Non-competitive inhibition
b) Reaction Rate
c) Co-factor and Co-enzyme
d) Active site
2. a) Give an overview of enzyme specificity and its different types.
b) What is the effect of pH on enzyme activity?
3. Explain ordered and random mechanism of bisubstrate enzyme reactions.
4. Differentiate between:
a) Fischer lock and Key hypothesis and Induced Fit Model
b) Hydrolases and Isomerases
5. Derive Alberty rate equation for bisubstrate enzyme catalyzed reactions.
PART-(B)
6. Explain the mechanism of chymotrypsin enzyme.
7. Write short note on the following:
a) Biotin
b) Tetrahydrofolate
c) Diagnostic Enzymes
d) Enzymes in baking industry
8. Discuss the Pyruvate Dehydrogenase complex and its mechanism of regulation.
9. Differentiate between the following:
i) Covalent and Ionic method of enzyme immobilization
ii) Concerted and Cooperative feedback inhibition
10. Draw a table specifying five marker enzymes, their organelle site and associated metabolic pathway.
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